Composite
pelB-rDAO

Part:BBa_K4225022:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-11)


pelB-rDAO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1070
    Illegal BamHI site found at 1998
    Illegal BamHI site found at 2252
    Illegal XhoI site found at 868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All parts are taken from biobricks and are codon optimized for our chassis, DH5α.